Insertion junction: CLIP2.004904_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.004904
Chromosome:chromosome 1
Location:6289317
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g044650 (1 of 1) PF00168//PF17047 - C2 domain (C2) // Synaptotagmin-like mitochondrial-lipid-binding domain (SMP_LBD) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACACGCGGACCCCTCCCATCCAAGTTACTCACTCCACCCATCCGCGGCC
Internal bar code:TTCGGAAAATGTCGCCTTGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3499
LEAP-Seq percent confirming:96.1039
LEAP-Seq n confirming:74
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:77

Suggested primers for confirmation by PCR

Suggested primer 1:CACAATCCGCATCATGCTGG
Suggested primer 2:AAAAAGGGACAATGTGCGGC