Insertion junction: CLIP2.004905_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.004905
Chromosome:chromosome 1
Location:5307467
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g037350 TEH2,CGLD2 Thioesterase family protein; (1 of 2) K17361 - acyl-coenzyme A thioesterase 9 [EC:3.1.2.-] (ACOT9) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCACCCACTCACCCACCCACCCAAGCACCCGCCCACCCAGGCACCCACC
Internal bar code:CGGTATGACGAATTCTACATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1526
LEAP-Seq percent confirming:18.1818
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGCAAACAGTGTCCCGTG
Suggested primer 2:TGTCACGTGTCTCTGAGCAC