Insertion junction: CLIP2.004911_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.004911
Chromosome:chromosome 9
Location:5796717
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g411200 PSB33,TEF5,LIL8 PSII assembly protein; (1 of 1) PTHR21496:SF0 - RIESKE DOMAIN-CONTAINING PROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCAGAAGGTTGCGAAGTCGTCGCAGGCGCGCGTGCTTGTGCCCGCTGG
Internal bar code:GTCGGTATCTCGCCGAAGTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:994
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGGATTCGATGCCGACAT
Suggested primer 2:GACACAGCTTCCAGAGGACC