| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.004913 |
| Chromosome: | chromosome 14 |
| Location: | 2059641 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621351 | (1 of 2) 2.7.6.5 - GTP diphosphokinase / Stringent factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGTCGGTGGCCCTGGGTGCACTGAGCTACAACCTCATGGCCACGCTGT |
| Internal bar code: | CCTTATTCATTACCTGTACATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 669 |
| LEAP-Seq percent confirming: | 69.2308 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAAAGCCAACGAAAACGG |
| Suggested primer 2: | CTTGCTGAAGGGTGCACAAC |