Insertion junction: CLIP2.004913_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.004913
Chromosome:chromosome 14
Location:2059643
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g621351 (1 of 2) 2.7.6.5 - GTP diphosphokinase / Stringent factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCACACAGCGTGGTAAACCAGAGGCTAGCGCCATGGTAGGAGCCTGTC
Internal bar code:TAGCACAGGCATTGCTAAGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:4176
LEAP-Seq percent confirming:80.7692
LEAP-Seq n confirming:63
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:78

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGCTGAAGGGTGCACAAC
Suggested primer 2:ACCAAAGCCAACGAAAACGG