| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004921 |
| Chromosome: | chromosome 7 |
| Location: | 3730976 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338000 | MCM2 | (1 of 1) K02540 - DNA replication licensing factor MCM2 (MCM2); Minichromosome maintenance protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGCGAAAAGCGCGCCAATAGGCAAGCCCTCCAAAGGCGCCTATAGTG |
| Internal bar code: | ATGGGCAAAGGCATCGGGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2815 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 124 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 124 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTCAGGAACGATGCAGG |
| Suggested primer 2: | GCCCCAGCAGCGATTTAATG |