| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.004958 |
| Chromosome: | chromosome 10 |
| Location: | 3251344 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g442650 | (1 of 1) PTHR10887:SF366 - DNA-BINDING PROTEIN-RELATED | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCCGAATGGGGCGACATGTGCTGGACTTCATTGGAGTGCAGGCCCCT |
| Internal bar code: | GTCTCTGATAAAATCGTTCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1818 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGCCCAACGACAATTTG |
| Suggested primer 2: | GATAATCAAGGCTCCCGGCA |