Insertion junction: CLIP2.004959_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.004959
Chromosome:chromosome 3
Location:145846
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g143947 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCTGACTCGGCACAGGCTGACGGTTGGCGACGATCGGAACACAAGCG
Internal bar code:CCCCGGAGAGGGGTGGCTGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:4791
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:103
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:103

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCCAGCTCCAGGTTGTAC
Suggested primer 2:CTTACACATGCGCGCTAACC