| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.004993 |
| Chromosome: | chromosome 7 |
| Location: | 3730971 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338000 | MCM2 | (1 of 1) K02540 - DNA replication licensing factor MCM2 (MCM2); Minichromosome maintenance protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAAGCCCGGCCCACTCTCAAATTCGCAGTCCTTCAGCCCAAAGGCTCG |
| Internal bar code: | ACTGGCTCTGCCTTTTCCTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1428 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCCAGCAGCGATTTAATG |
| Suggested primer 2: | AAGGTCAGGAACGATGCAGG |