| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005008 |
| Chromosome: | chromosome 3 |
| Location: | 1944714 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155250 | (1 of 1) IPR002202//IPR029058 - Hydroxymethylglutaryl-CoA reductase, class I/II // Alpha/Beta hydrolase fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATCTCGCCCGCGCCGTCATCTGCGTCGGTTGTGTTGCCGCCGGGCCGC |
| Internal bar code: | TATCCACCTAAAGGTCGGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1344 |
| LEAP-Seq percent confirming: | 52.6316 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCGTATGTCCCAGGAGG |
| Suggested primer 2: | GATACCATCCCTTCGGCCAG |