| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.005009 |
| Chromosome: | chromosome 3 |
| Location: | 741818 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145987 | (1 of 7) IPR000104//IPR020683 - Antifreeze protein, type I // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCTCTAAAGGTTGTTGTGGCGGCGGCGGCGGCGGCGGCTGTGACAGC |
| Internal bar code: | GTACTAGGTGACAAGCTAATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 189 |
| LEAP-Seq percent confirming: | 4.34783 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAATGTGCTACAGCCAGG |
| Suggested primer 2: | TAGGGTTGTCAGTTCGGCAC |