| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005014 |
| Chromosome: | chromosome 16 |
| Location: | 4371985 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668650 | (1 of 1) PTHR34204//PTHR34204:SF2 - FAMILY NOT NAMED // RNA-BINDING ASCH DOMAIN PROTEIN | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGTCGGGTCATTTGTTGATTTGTCGAGCCGACCGCCCGGCCAAAAGC |
| Internal bar code: | AGTACCTGGCTCCAGAGCCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1239 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGAGTGGATTAAACAGCG |
| Suggested primer 2: | ATGTGCATTACCACTGCCGA |