| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.005020 |
| Chromosome: | chromosome 4 |
| Location: | 645345 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217946 | (1 of 1) PTHR22769:SF35 - NUDIX HYDROLASE FAMILY PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGTACGCTGGGTTTATGTAGTCGCTCGAGGATGGCGACGGGCCGGGCG |
| Internal bar code: | TGTAAAAGGACCCGGGGGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1581 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGCATTATCCCTTGCTTG |
| Suggested primer 2: | AACAGAGCGAGGTTAGCACC |