| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005033 |
| Chromosome: | chromosome 5 |
| Location: | 2346252 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238322 | TSW2 | (1 of 2) 6.1.1.2 - Tryptophan--tRNA ligase / Tryptophanyl-tRNA synthetase; Putative tryptophanyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTCAAAGGTAGGGGAGTGAGTGGGTAGTGGGGGCGTGGGCGCGTGTAA |
| Internal bar code: | TGGGAAAGTTAAGATTAAGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 274 |
| LEAP-Seq percent confirming: | 19.7917 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTATTCCTGCCCGTTTCCC |
| Suggested primer 2: | TAGGTCGAGGGGGAAAGAGG |