Insertion junction: CLIP2.005036_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.005036
Chromosome:chromosome 1
Location:2510032
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g014800 MSH7,CPL21 putative MutS type 2 DNA repair protein; (1 of 1) K07456 - DNA mismatch repair protein MutS2 (mutS2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCGTCGTCATCATCGCCCACGTAGCCGCGCCCGCGCAGCGCTGCCACC
Internal bar code:ATCGGATACAAAATTAAACAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:86
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACCAATTGCCTCTAGGGTGC
Suggested primer 2:CTCCTCAACACTGGTGCCAT