| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.005047 |
| Chromosome: | chromosome 2 |
| Location: | 6319425 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387550 | CGL142 | Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGAGGGCACGGACGGTACGCTGGAAGAAGGTGTCTCATCCGTCGTGCT |
| Internal bar code: | CCGGCTCATCTAGCAACGCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1441 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCTTGGCATGCTCTGTA |
| Suggested primer 2: | GTACGGTCGGGCGCTTAATA |