Insertion junction: CLIP2.005052_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.005052
Chromosome:chromosome 12
Location:9006620
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g545101 XDH1 (1 of 1) 1.17.1.4 - Xanthine dehydrogenase / Xanthine/NAD(+) oxidoreductase; Xanthine dehydrogenase/oxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCTGCTGCACTCGGACTGCTGCTACAAGGTTCCGCACATGCGCGTGC
Internal bar code:ATACCTTGAGTTAGTCCTAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2188
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTGCTCCATCACCTGAGG
Suggested primer 2:CACACCATGCACACACACAG