| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005054 |
| Chromosome: | chromosome 16 |
| Location: | 4840197 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687300 | CPLD61 | Conserved in the Plant Lineage and Diatoms; (1 of 3) PF13599 - Pentapeptide repeats (9 copies) (Pentapeptide_4) | 5'UTR |
| Cre16.g687350 | ACO3,ACX3 | (1 of 1) PTHR10909:SF11 - ACYL-COENZYME A OXIDASE-LIKE PROTEIN; Acyl-CoA oxidase/dehydrogenase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATATGGTAGCTGGGAGGCGTCCAGCTGAGGACATTCGGCACCCGCAAT |
| Internal bar code: | TTTCTTGATATTGTTAGACCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1794 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTGGTGAAGAAAGCGGA |
| Suggested primer 2: | TACAGGTGTGGTACGTTGGC |