Insertion junction: CLIP2.005060_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.005060
Chromosome:chromosome 6
Location:7588457
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g301800 MFT19 Major facilitator superfamily transporter; (1 of 2) PF05977 - Transmembrane secretion effector (MFS_3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGGCAGCAACACGCTCTATATCCACTCCTTCGCCATCCTGCAGCACCG
Internal bar code:AGTTGGCCTGACCAGTTAACCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2266
LEAP-Seq percent confirming:96.4912
LEAP-Seq n confirming:55
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTACACGTGCTATGTGCG
Suggested primer 2:GACACGAAAAACTGGCACCC