| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005061 |
| Chromosome: | chromosome 9 |
| Location: | 1083772 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401850 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCGCATCGCATCCCTCCGCGTCTGGCCGCTGCCTGTCCGCATGAGCGT |
| Internal bar code: | GTGGCGGTCAGAAGGGGGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3024 |
| LEAP-Seq percent confirming: | 87.7551 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCACGTTGAGACCTTCCA |
| Suggested primer 2: | CCCTGGCTAATGAAATGCGC |