| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.005072 |
| Chromosome: | chromosome 3 |
| Location: | 8717034 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g208721 | CGL92 | (1 of 2) 2.7.6.5 - GTP diphosphokinase / Stringent factor; predicted protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGATTGCAGTTGTTGTCAATTGTGTGTGTGTGGTCAGGAAACCGGCTG |
| Internal bar code: | ATGTACTTTGTAACTTTCCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 134 |
| LEAP-Seq percent confirming: | 78.9474 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCCATTGTGCCAGTTTG |
| Suggested primer 2: | GTATTTGTGGTGGGGAGCGA |