Insertion junction: CLIP2.005100_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.005100
Chromosome:chromosome 1
Location:4688314
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g032450 (1 of 4) K06816 - golgi apparatus protein 1 (GLG1, ESL1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCGCTGCTGGCGGCGTGCGCGCACGACATCATCACCCACTGCGACTA
Internal bar code:TAATCGCGAAAACAGATACCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3142
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:GCGATGTGCACTACCTTCCT
Suggested primer 2:GAAACCTGGGCTGTCGAGAA