| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005101 |
| Chromosome: | chromosome 6 |
| Location: | 2420853 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268750 | MME1,MME | (1 of 1) 1.1.1.39 - Malate dehydrogenase (decarboxylating) / Pyruvic-malic carboxylase; Malate dehydrogenase 1, decarboxylating | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAAACAAAGAATTTCACCCCTCCAGTCCCAATCGCCCAACCAAGCAT |
| Internal bar code: | CTAAGGACGTAGGCGATTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1052 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCAAGAGAGAGGAGGAG |
| Suggested primer 2: | CTGGACGCGCAACATTGAAA |