| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.005113 |
| Chromosome: | chromosome 1 |
| Location: | 315554 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001750 | TIG1 | chloroplast trigger factor; (1 of 1) PTHR30560 - TRIGGER FACTOR CHAPERONE AND PEPTIDYL-PROLYL CIS/TRANS ISOMERASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGGGGCGATGCTGCATGGTGCATGGCGCGGCAGCGTGGCGTGGGGTG |
| Internal bar code: | TAAATCGAAGCTTCGTAACTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2074 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGGGGCCGAGTATAAGT |
| Suggested primer 2: | TTTACGCGACGCCTCTATCC |