| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005125 |
| Chromosome: | chromosome 4 |
| Location: | 3118489 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225800 | CPLD71 | dihydrolipoyllysine-residue acetyltransferase; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGGGCGCCGCCGCAGCCAGCAGGCAGCTCCACACGGTGGCCGCCGGG |
| Internal bar code: | GAATGGGTGGGTTCGGGACGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5768 |
| LEAP-Seq percent confirming: | 64.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTAAGCTGGGGAAGGCAG |
| Suggested primer 2: | TTGCAGGTCCATGATTGCCT |