Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.005130 |
Chromosome: | chromosome 11 |
Location: | 320910 |
Confidence (%): | 63 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g467569 | ATPD,ATPD1 | Chloroplast ATP synthase delta chain; (1 of 1) K02113 - F-type H+-transporting ATPase subunit delta (ATPF1D, atpH) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGTCCACAGCAGCGCGTATTGTAGCAATGTCCAAATTCCCGGACGAA |
Internal bar code: | ATTATTGGAAGGTGTGGCCACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 147 |
LEAP-Seq percent confirming: | 45.4545 |
LEAP-Seq n confirming: | 10 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCCCCCATAAGCTTGCTGG |
Suggested primer 2: | CGTTGTGGAGTCGGACAAGA |