Insertion junction: CLIP2.005133_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.005133
Chromosome:chromosome 2
Location:8275309
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g143400 PDE27 (1 of 1) K18437 - high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8 (PDE8); 3'%252C5'-cyclic-nucleotide phosphodiesterase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGATCATTGTGACGCTTGGGGAAGCGCGCATCGGAGTCACTTGCTCGTCC
Internal bar code:AAAGTATAATTGTAGTAGATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2429
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TTCACACCAGCCAGCTCAAA
Suggested primer 2:CCCTGCAATGCGAACACAAA