| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005135 |
| Chromosome: | chromosome 9 |
| Location: | 2029085 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396650 | PAT2 | Phosphate acetyltransferase; (1 of 2) 2.3.1.8 - Phosphate acetyltransferase / Phosphotransacetylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTGGGGGGGCTCAGCCCAAACAAGGGGTGTCAGCTTTGTACAGCTGG |
| Internal bar code: | TAAACCCTAGTATACGCAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3503 |
| LEAP-Seq percent confirming: | 83.75 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAACTATGTTGGCGGCAA |
| Suggested primer 2: | CACAACCACGCAAGACAAGG |