| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005142 |
| Chromosome: | chromosome 12 |
| Location: | 2111540 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g510034 | (1 of 8) PF13432 - Tetratricopeptide repeat (TPR_16) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTTCCCTGATTCTGCCTGCTCCTGGCCCTGAGCCTGTGGGGCAACAGC |
| Internal bar code: | TTCGGGGTTCGTCCTAGTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 722 |
| LEAP-Seq percent confirming: | 15.7895 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTTAATGCTGTCGCGAG |
| Suggested primer 2: | GTTACAACAACTGCCAGCCG |