Insertion junction: CLIP2.005149_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.005149
Chromosome:chromosome 10
Location:1732075
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g430150 REP27,LPA1 (1 of 1) PF11998//PF13414 - Protein of unknown function (DUF3493) (DUF3493) // TPR repeat (TPR_11); Low Photosystem II Accumulation 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGAAGCAACAGGCAGTCCCCATTTTGTTTCTGAAGGGGCGGCAGTTTT
Internal bar code:TGATACGGCGGAACTTCGACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:7006
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGGGAATGTTGCACTTT
Suggested primer 2:GTATTTGTGTGCGGGAGGGA