| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005166 |
| Chromosome: | chromosome 11 |
| Location: | 4182535 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g481450 | ATPG1,ATPG | Chloroplast ATP synthase subunit II; (1 of 2) K02109 - F-type H+-transporting ATPase subunit b (ATPF0B, atpF) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGACCCTGCCCGTCATGGCCGGCGAGTTCCTGCTGCTGATGGTGTTC |
| Internal bar code: | CTTTCGCATTCATTAGGATTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2267 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGATAACCACGCGCAAG |
| Suggested primer 2: | ATTAGTGGGTCGACGCGTAC |