| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.005179 |
| Chromosome: | chromosome 13 |
| Location: | 1997686 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576650 | HIS4 | Bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase; (1 of 1) 3.6.1.31 - Phosphoribosyl-ATP diphosphatase / Phosphoribosyladenosine triphosphate pyrophosphatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAGGGTTTTAGAACAGCAGAACAGAAACCGTGAAAGCGTGAAAGCGTG |
| Internal bar code: | GCGACTTCCGCCTCTCCAAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 529 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGATACCGCCACATCCGC |
| Suggested primer 2: | ATTCTTATACCAGCGGCGGG |