| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005180 |
| Chromosome: | chromosome 6 |
| Location: | 5535639 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286800 | HBP1 | Histone gene transcript 5' hairpin-binding protein; (1 of 1) K18710 - histone RNA hairpin-binding protein (SLBP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTCCAATGGATATGGCGGGTGCTGCGCCACTGCATATGCCGCTAGTCC |
| Internal bar code: | CGTTTGCCCTCTCTTTTATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 557 |
| LEAP-Seq percent confirming: | 41.4634 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTGTTAACCTGCTCCTGG |
| Suggested primer 2: | CCCGATGGCGCATTCTCTAT |