| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005182 |
| Chromosome: | chromosome 9 |
| Location: | 6680017 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g416750 | CCT2 | T-complex protein 1, beta subunit; (1 of 1) K09494 - T-complex protein 1 subunit beta (CCT2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTGCACTGTGCACGCATCAGTTGTTTTGCTCATTCCTGACACTGCAC |
| Internal bar code: | ACAGGTTTAGCTCGCGTAAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4444 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 119 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 119 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAAAAGAGCTGGGGAGCCG |
| Suggested primer 2: | TATACAACGGACGCGGATGG |