Insertion junction: CLIP2.005188_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.005188
Chromosome:chromosome 12
Location:481081
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g494550 RNP10 RNA binding protein; (1 of 3) K14947 - epithelial splicing regulatory protein 1/2 (ESRP1_2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAGAAGGACCGCGCCAAGTTCGGGCCGGAGTACGGCGACCGCTTCTGC
Internal bar code:TACGTCCTAGTAACGTGATACT

Confirmation - LEAP-Seq

LEAP-Seq distance:2585
LEAP-Seq percent confirming:43.4783
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTGCACACAAATCCAGGC
Suggested primer 2:GTTTGGGCTCAAGCTTTGGG