| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.005191 |
| Chromosome: | chromosome 17 |
| Location: | 968420 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g703000 | (1 of 5) 3.2.1.3 - Glucan 1,4-alpha-glucosidase / Lysosomal alpha-glucosidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGCCGTACAAAGCGTGGAACGGCGCCGCATTGCCACTACTGGTACTG |
| Internal bar code: | GAACAGAGACTGGCGCCGTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1391 |
| LEAP-Seq percent confirming: | 30.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCCCACCAACACCATCA |
| Suggested primer 2: | AACACGGTGGCTACTTCCTG |