| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.005200 |
| Chromosome: | chromosome 10 |
| Location: | 5776067 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g460050 | FLU1,FLU,FLP | (1 of 1) PTHR10098//PTHR10098:SF123 - RAPSYN-RELATED // SUBFAMILY NOT NAMED; FLU protein, chloroplast precursor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGAAGAACGGCGCCGCGGCCATGGTGCGCTTCGAGAAGGCGCTCATGC |
| Internal bar code: | ATTCCTGAACAAACTTGAGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 428 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGTACTCGCCGATCTCC |
| Suggested primer 2: | TTCAGCATGGAGGAGGAGGA |