| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.005207 |
| Chromosome: | chromosome 12 |
| Location: | 6943401 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g561450 | (1 of 1) PTHR11228//PTHR11228:SF23 - RADICAL SAM DOMAIN PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAATGCCGCACTGACATGAGGCCACAGGGCACACCGTGCGTCCCCCCA |
| Internal bar code: | TGACGGGAATGTTAATTTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2465 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGTCCGCCTACAAAGCAG |
| Suggested primer 2: | CAGCCAAAGCAGTGGTTGTC |