Insertion junction: CLIP2.005216_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.005216
Chromosome:chromosome 16
Location:1404855
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g652200 MMP9 Metalloproteinase of VMP family; (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGATTTCATGCGGACAGACGGAGGCGAGGAGGCGGGGAGGCGACCCAG
Internal bar code:GAGGGCCGTCCACATGGCACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:3411
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCACGCCAGTAGCTATGG
Suggested primer 2:AGGACTTGACGACTTGCCAG