Insertion junction: CLIP2.005227_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.005227
Chromosome:chromosome 4
Location:1685346
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g212401 UBC15 (1 of 2) K10586 - baculoviral IAP repeat-containing protein 6 (apollon) (BIRC6, BRUCE); E2 Ubiquitin conjugating enzyme CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCAGCGCGAGGCGGCGGCGTGCGCGGAGGCGGCGAAGGAGGCGGAGG
Internal bar code:CGAAGAAAGTAGACGCAGTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:153
LEAP-Seq percent confirming:18.1818
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:ACATAGGTGGACAGCTTGGC
Suggested primer 2:CATGATCCTAGTGGCCGACC