| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.005227 |
| Chromosome: | chromosome 4 |
| Location: | 1685373 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212401 | UBC15 | (1 of 2) K10586 - baculoviral IAP repeat-containing protein 6 (apollon) (BIRC6, BRUCE); E2 Ubiquitin conjugating enzyme | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGCGCGGCGTAGTGTCGGCGCAGCACGTCGGCAAAGGTGGCGCCACC |
| Internal bar code: | AACAAGATTGATTTTCCGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 651 |
| LEAP-Seq percent confirming: | 65.625 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGATCCTAGTGGCCGACC |
| Suggested primer 2: | ACATAGGTGGACAGCTTGGC |