Insertion junction: CLIP2.005245_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.005245
Chromosome:chromosome 12
Location:8559541
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g548950 LHCBM7 (1 of 3) K08913 - light-harvesting complex II chlorophyll a/b binding protein 2 (LHCB2); Light-harvesting Chlorophyll a/b binding protein of LHCII 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCCGTGTTACACAACAAGGGCAAATCGCAAACCAACATACCAGTGGC
Internal bar code:GTGGAATCAACCAGTTCTGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:3171
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:61
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:GGCAACGAGAACCTGATCCA
Suggested primer 2:GAGACCGCCTCCGTGATTAG