| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005246 |
| Chromosome: | chromosome 12 |
| Location: | 495708 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g494350 | EMP70 | Endomembrane protein 70; (1 of 1) PTHR10766:SF54 - ENDOMEMBRANE FAMILY PROTEIN 70 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGTACTCGTAAGGCAGCTCCGTGTCAAACGAGGTCAGCGTGCTCACGT |
| Internal bar code: | CGGGGCCTTCAACTGTTAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1517 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGAGAGTGCGACAGTGTC |
| Suggested primer 2: | CGTCAATAAGCCGCTTCAGC |