Insertion junction: CLIP2.005257_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.005257
Chromosome:chromosome 1
Location:807779
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g004450 CPLD42 (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517); conserved expressed membrane protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCACCACCGCCAAAGCCGAAGAAGTACACCGAAGGCCGAGCAACGGCCT
Internal bar code:CAGTCTCTGGTTTTGTCTCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2097
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:AGAAGAACGGCGTTGTTTGC
Suggested primer 2:TGCAACATAATCCACCGGCT