| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005278 |
| Chromosome: | chromosome 8 |
| Location: | 3468304 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378650 | CGl131 | (1 of 18) IPR011051 - RmlC-like cupin domain; Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTCCCCTTGTCCAGCACGAGCTGCGCGTCCTCCTTATCGAACATCTT |
| Internal bar code: | GGCATGTGTTCTCTCCCAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3083 |
| LEAP-Seq percent confirming: | 88.6364 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCACCAACTCGGAGGCAG |
| Suggested primer 2: | AACGACCTTGACGGACACTC |