| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.005283 |
| Chromosome: | chromosome 7 |
| Location: | 5935776 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g354250 | PGK2 | (1 of 2) PF00162 - Phosphoglycerate kinase (PGK); Phosphoglycerate kinase | 3'UTR |
| Cre07.g354301 | RDP1 | Putative rhodanese domain phosphatase; (1 of 3) K18065 - Cdc25 family phosphatase [EC:3.1.3.48 1.20.4.-] (CDC25) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCAGTTACAAATCTTACTCAGGGAGCTTGCAAGAACCTGTACCAGTT |
| Internal bar code: | CCTTTCTACCTCTGTTCCAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 48 |
| LEAP-Seq percent confirming: | 3.125 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACGGGTATCCTCGAATGG |
| Suggested primer 2: | GGTGGAGCTGGAGGTAGGTA |