| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005294 |
| Chromosome: | chromosome 7 |
| Location: | 335784 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314450 | SMM22,CGL33B | (1 of 1) PTHR12176:SF19 - S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN; conserved methyltransferase of unknown function | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACAAAGGTGTGTGCGCGTGCGCGTGCGCGTGTGCATGTGTGTGAACA |
| Internal bar code: | CAGCGGGCAGTGGGCGGAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2468 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACAGCGTTGGAAGCATGA |
| Suggested primer 2: | CCCTTCACTGCATGTACCGT |