| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.005321 |
| Chromosome: | chromosome 3 |
| Location: | 589710 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145387 | FAP239 | Coiled-Coil Flagellar Associated Protein 239 | 5'UTR |
| Cre03.g145407 | (1 of 2) IPR000571//IPR002110//IPR020683 - Zinc finger, CCCH-type // Ankyrin repeat // Ankyrin repeat-containing domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCTCGTTTAGAATGGAGTCGTCGGGAGTGCCTCCCGGGAACGGGGCT |
| Internal bar code: | ATGTCAGAATCCTCTATGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1363 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGACCTGCTCTGAACAAC |
| Suggested primer 2: | CGTCCCTCGTATGTGAGCTC |