| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.005325 |
| Chromosome: | chromosome 17 |
| Location: | 4597228 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732250 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTTCGCTGTCAATCTTTCTACACCTGGCTCTCGCCGTCGCAGCTCCCT |
| Internal bar code: | TTCCTCTCGCTCGAGATACTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2342 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCTGGTTACAGGATGGTT |
| Suggested primer 2: | GTACCCTGCCATGCTGTCTT |