| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.005327 |
| Chromosome: | chromosome 16 |
| Location: | 2608120 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661150 | CGL5 | Conserved in the Green Lineage; (1 of 2) 5.2.1.14 - Beta-carotene isomerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGTGCAAGGCTGTCTGGCAGCAACACAGGCCACGGTTCAAAGCACTGT |
| Internal bar code: | CTTCGTCAGAGACCGCGAAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1396 |
| LEAP-Seq percent confirming: | 70.5882 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACATTCCGGTCCCAATGG |
| Suggested primer 2: | CCTCCTTAGGCATGAGCGAG |